Microvesicles from mesenchymal stem cells enhance frozen sperm

http://www.ncbi.nlm.nih.gov/pubmed/23958892

Reprod Toxicol. 2013 Aug 16. pii: S0890-6238(13)00330-4. doi: 10.1016/j.reprotox.2013.07.024. [Epub ahead of print]

Mesenchymal stem cell derived microvesicles: Trophic shuttles for enhancement of sperm quality parameters.

Mokarizadeh A, Rezvanfar MA, Dorostkar K, Abdollahi M.

Source

Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.

Abstract

Diminishing sperm quality during cryopreservation process ends up in a complete or partial loss of sperm's fertilizing potential. Rehabilitation of such affected sperm is crucial to improve their fertilizing potential. A variety of evidence has indicated that secreted microvesicles (MVs) from mesenchymal stem cells (MSCs) are involved in regeneration of injured endogenous cells via shuttling MSC trophic molecules. Sperm obtained from cauda epididymides of adult male Wistar rats were equally assigned to four separate groups. Following suspension in cryoprotectant extender, sperm were untreated or treated with increasing concentrations of MSC-derived MVs (25, 50 and 100μg). After incubation in successive steps, sperm were cryopreserved. The frozen-thawed sperm were assessed for viability, motility and antioxidant capacity parameters. Consequently, expression levels of surface adhesion molecules (CD29, CD44, ICAM-I and VCAM-I) involved in sperm fusogenic and signaling properties, were assessed by flow cytometry. Results showed an enhanced quality parameters and adhesive properties of cryopreserved sperm following treatment with MSC-derived MVs.

Small dose (100 micrograms) of exosomes (cell derived vesicles) from mesenchymal stem cells improved functional recovery and enhanced neurite remodeling, neurogenesis, and angiogenesis in a rat stroke model.  A single treatment given by tail vein.
http://www.ncbi.nlm.nih.gov/pubmed/23963371

Systemic administration of exosomes released from mesenchymal stromal cells promote functional recovery and neurovascular plasticity after stroke in rats.

J Cereb Blood Flow Metab. 2013 Aug 21. doi: 10.1038/jcbfm.2013.152.

Attended great mesenchymal stem cell conference this week with Dr. Arnold Caplan, the first to discover and describe this cell type.

MSC 2013 - Adult Stem Cell Therapy and Regenerative Medicine

www.mscconference.net/

The Science of Mesenchymal Stem Cells and Regenerative Medicine - Arnold Caplan PhD (Part 3)

In Part 3, Dr. Caplan discusses the science behind mesenchymal stem cells: sources of mesenchymal stem cells (MSCs), the fact that all MSCs are pericytes so one can find them in any tissue that has blood vessels, pericytes express markers of MSCs, frequency of pericytes in human tissue, most abundant source of pericytes is adipose (fat) tissue, adipose-derived stem cells, how MSCs are separated from fat, chemistries MSCs from different tissues are not the same, MSCs function at sites of injury, mesenchymal stem cell homing in mice, MSCs don't make fat, they don't make muscle but they do come back as pericytes, and not all pericytes are MSCs.