http://www.ncbi.nlm.nih.gov/pubmed/23958892
Reprod Toxicol. 2013 Aug 16. pii: S0890-6238(13)00330-4. doi: 10.1016/j.reprotox.2013.07.024. [Epub ahead of print]
Mesenchymal stem cell derived microvesicles: Trophic shuttles for enhancement of sperm quality parameters.
Source
Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Abstract
Diminishing
sperm quality during cryopreservation process ends up in a complete or
partial loss of sperm's fertilizing potential. Rehabilitation of such
affected sperm is crucial to improve their fertilizing potential. A
variety of evidence has indicated that secreted microvesicles (MVs) from
mesenchymal
stem cells (MSCs) are involved in regeneration of injured endogenous
cells via shuttling MSC trophic molecules. Sperm obtained from cauda
epididymides of adult male Wistar rats were equally assigned to four
separate groups. Following suspension in cryoprotectant extender, sperm
were untreated or treated with increasing concentrations of MSC-derived
MVs (25, 50 and 100μg). After incubation in successive steps, sperm were
cryopreserved. The frozen-thawed sperm were assessed for viability,
motility and antioxidant capacity parameters. Consequently, expression
levels of surface adhesion molecules (CD29, CD44, ICAM-I and VCAM-I)
involved in sperm fusogenic and signaling properties, were assessed by
flow cytometry. Results showed an enhanced quality parameters and
adhesive properties of cryopreserved sperm following treatment with
MSC-derived MVs.
